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Cryomacroscopy of Vitrification:

Selected Experiments on DP6 and VS55

 

The objective for this web page is to provide a selection of images of physical events such as crystallization and vitrification, and to serve as an appendix for the following two publications:

1.      Rabin, Y., Taylor, M.J., Walsh, J.R., Baicu, S., Steif, P.S. (2005): Cryomacroscopy of vitrification, Part I: A prototype and experimental observations on the cocktails VS55 and DP6, Cell Preservation Technology, 3(3):169-183 Article (PDF 1.3MB)

2.      Steif, P.S., Palastro, M., Wen, C.R., Baicu, S., Taylor, M.J., Rabin, Y. (2005): Cryomacroscopy of vitrification, Part II: Experimental observations and analysis of fracture formation in vitrified VS55 and DP6, Cell Preservation Technology, 3(3):184-200 Article (PDF 1.6MB)

 

Related work: Cryomacroscopy of fracture formation in vitrified thin films of cryoprotectants

 

 

 

Cooling Rate

Temperature Range

H1

-40⁰C to -100⁰C

H2

-100⁰C to -130⁰C

H3

-130⁰C to -100⁰C

H4

-100⁰C to -40⁰C

 

Exp.

H1 ⁰C/min

H2 ⁰C/min

H3 ⁰C/min

H4 ⁰C/min

Tmin

 ⁰C

 

DP6 #41

100.0

106.0

-14.0

-300.0

-156.6

Fractures during cooling followed by recrystallization

DP6 #40

97.3

100.0

-26.5

-400.0

-161.1

Fractures during cooling followed by recrystallization

DP6 #39

80.0

81.8

-26.0

-360.0

-193.7

Fractures during cooling followed by recrystallization

DP6 #31

32.7

31.0

-6.9

-3.5

-158.1

Fractures during cooling followed by recrystallization

DP6 #55

21.2

19.2

-6.1

-8.7

-146.1

Fractures during cooling followed by recrystallization

DP6 #32

17.4

16.1

-4.4

-2.3

-150.6

Fractures during cooling followed by recrystallization

DP6 #54

16.7

14.2

-47.4

-171.4

-142.7

Fractures during cooling (no rewarming above -100⁰C)

DP6 #34

6.1

3.4

-13.2

-9.8

-129.9

Fractures during cooling followed by recrystallization

DP6 #74

4.8

1.1

-18.7

-277.0

-129.6

Fractures during cooling followed by recrystallization

DP6 #36

11.5

7.6

-15.0

-10.0

-136.1

Vitrification followed by recrystallization

DP6 #35

4.4

2.1

-14.3

-9.6

-133.2

Vitrification followed by recrystallization

DP6 #42

2.4

1.5

-14.3

-40.0

-135.7

Vitrification followed by recrystallization

DP6 #43

14.3

11.8

-18.6

-46.2

-133.0

Crystallization followed by recrystallization

DP6 #93

8.4

5.1

-12.1

-29.8

-131.4

Crystallization followed by recrystallization

DP6 #94

7.2

3.4

-13.9

-29.4

-130.6

Crystallization followed by recrystallization

DP6 #82

5.8

2.8

-15.6

-50.7

-128.1

Crystallization followed by recrystallization

DP6 #33

3.5

1.5

-12.6

-9.1

-129.2

Crystallization followed by recrystallization

VS55 #69

5.3

0.9

-15.5

-30.5

-130.6

Fractures during cooling followed by recrystallization

VS55 #60

3.1

0.5

-12.8

-19.0

-131.0

Fractures during cooling followed by recrystallization

VS55 #86

14.3

10.0

-18.7

-53.4

-131.9

Vitrification followed by recrystallization

VS55 #71

7.9

4.1

-16.7

-3.5

-130.7

Vitrification followed by recrystallization

VS55 #101

3.8

2.6

-6.1

-13.0

-131.0

Vitrification followed by minor recrystallization

VS55 #107

3.2

1.9

-5.5

-10.4

-131.1

Vitrification followed by minor recrystallization

 

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This research has been supported, in part, by the National Heart Lung and Blood Institute, NIH Grant # 1R01HL069944

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