Biochemistry I Fall Term, 2005

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Your "assignment" is to purify the enzyme "BC-1ase". It is an enzyme found in the cytosol of yeast cells. Its molecular weight is 25,000 Da; its pI = 5.1; and its absorption coefficient = 0.65 at 280 nm (1mg/mL solution). This protein is responsible for the synthesis of ATP in the cell from AMP and inorganic phosphate.



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Lecture 20: Protein Purification.