|Biochemistry I - Activity of PFK +/- AMP: PFK-DL1
(Each calculated vo value has a small "experimental error" added to it.)
In the following dry lab you will measure the activity of PFK as a function of ATP concentration in the absense or presence of AMP.
The amount of F6P (substrate) will be fixed at 100 uM.
- Before starting, review the reaction catalyzed by PFK in glycolysis. What are the substrates and what are the products?
- Vary [ATP] and record the initial velocity in the absence of AMP (left box) and in the presence of 25 uM AMP (right box). Plot the initial velocity versus [ATP].Use ATP levels from 0 to 700 uM, in steps of 50 uM for the first few points, then steps of 100, and then steps of 200, e.g. 0, 50, 100, 150, 200, 300, 400, 600 ...