Human Ribonuclease

How to identify active site residues:
  1. They should be conserved in the sequence of the enzyme from all species.
  2. They should be close in space
  3. They should account for the known data (e.g. pH effect on kcat or KM.

Use the following to display residues of the same type:




Use the following to display individual residues
(Note: only residues 7 or higher are present in this structure)
1. Enter a residue number in this box (do not hit return):
Residue =
2. For the above residue, Display it or Label it
You can display more than one residue by using the console:
  1. Right click over the JMol window, a pop-up menu should appear.
  2. Select "Console", a box with yellow borders should appear.
  3. In the lower text window type the following to display residues 29, 45, and 57:
    1. select 29,45,57
    2. wireframe 0.6
    3. Color CPK
  4. To remove the wireframe, enter "wireframe off"
You can label your selected residues as follows:
  1. Open the console if it has been closed
  2. Select (29, 45, 57) and *.ca
  3. label %n%R
  4. color labels black
To Export an image:
  1. Right click to bring up the menu
  2. Go to "File" and then "Export"