Biochemistry 03-232 - Dry Lab

Protein Unfolding - Protein A and B

Protein A has an enthalpy of +100 kJ/mol
Protein B has an enthalpy of +200 kJ/mol

Enter a temperature:
T = K (273-373K)
For the above temp, Calculate the fraction unfolded
Protein A - FU =
Protein B - FU =
There is a small amount (5%) of experimental error.
Only use values of fraction unfolded that range from 0.2 to 0.8 if you are determining the enthalpy from the van't Hoff plot.