42-101
Intro to BME (Przybycien)
Spring
2005
PROBLEM
SET No. 2
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Posted: |
1/19/05 |
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Due: |
1/28/05 in class |
Problems for grading:
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1. |
Units in equations revisited. Our blood volume correlation based on a person’s size is given, for males, by: Vblood = aH3 + bM + c, where Vblood is the blood volume in liters, H is the person’s height in centimeters, and M is the person’s mass in kilograms. a. Modify the correlation, i.e. determine new values of a, b and c, so that values of H in feet and M in lbm can be plugged in to obtain values of Vblood in pints. b. If a man weighing 215 lbm with a height of 6 ft 1 in gives a pint of blood at a donation center, approximately what percent of his total starting blood volume has the man donated? c. The heights of 35 male students in 42-101 Intro to BME were measured and were determined to be 5.71±0.25 feet (mean±one std deviation). The masses of 17 male students in 42-401 BME Design were measured and were determined to be 172±15 lbm (mean±one std deviation). Estimate the average blood volume of male students in biomedical engineering classes (in liters) using this data and report with estimated 95% confidence limits. Can you identify any flaws in this analysis? What would you do differently? |
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2. |
Domach Text problem 1.2 |
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3. |
Domach Text problem 1.5 |
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4. |
Domach Text problem 1.7 |
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5. |
Domach Text problem 1.11 |
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6. |
EMP1 is a 20 amino acid peptide that has a biological activity the mimics that of erythropoetin (EPO) – a billion+ dollar per year drug produced by Amgen. EPO is used clinically to stimulate red blood cell production – hence its misuse in doping by athletes. If we were to make this peptide in Saccharomyces cerevisiae using recombinant DNA technology, we would transfect the yeast with a plasmid containing a gene coding for EMP1. What best putative sequence of nucleotides would you use in this gene to code for EMP1? Justify your selection. You may ignore promoter sequences and the Shine-Delgarno box. Useful web links…. EMP1 amino acid sequence and background information from Johnson et al.,
Biochemistry. 1998 Mar 17;37(11):3699-710. S. cerevisiae
codon usage preferences see
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Updated
on 1/19/05 by TMP