Feedback for Multiple Choice Quiz 36

  1. DNA primase functions only to initiate replication. RNA polymerase functions primarily to transcribe DNA, but can also serve to synthesize RNA primers for DNA replication.

  2. Okazaki fragments are made on the lagging strand; DNA ligase only joins DNA; helicase + ATP unwinds DNA ahead of the replication forks.

  3. This is the normal biological role of DNA ligase.

  4. The two exonuclease activities are coupled during the repair of gapped DNA; one degrades DNA in a 3' --> 5' direction; the other in the 5' --> 3' direction; the latter activity is essential for E. coli viability.

  5. This important difference makes PolI and essential enzyme in DNA replication.

  6. Dna B is responsible for unwinding the DNA, this causes a tightening of the double helix in front of the replication fork. This tightening, or overwinding, is removed by gyrase.
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