Feedback for Multiple Choice Quiz 35

  1. Plasmids are circular and must contain an origin of replication if they are to be replicated in the bacterial cell. The antibiotic resistance is used to maintain the plasmid in the cell. In addition, an expression vector has to be able to produce mRNA that will encode the desired protein.

  2. The point of hydrolysis is at the 3'-OH. Since the enzyme cuts both strands, there at two 3'OH and two 5'Phosphate ends.

  3. DNA ligase uses ATP (or NAD in the case of some ligases) to rejoin the 3-OH to the 5-Phosphate.

  4. None of the DNA polymerases synthesize in the 3' to 5' direction.

  5. The only difference between the two techniques is the nature of the polymer. In the case of proteins, we coat the proteins with SDS to give them a uniform charge to mass ratio. DNA comes that way naturally!
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