Feedback for Multiple Choice Quiz 34
  - TM is a generic term refering to the half-way point of a melting curve. 
- TM = 81.5 + 0.41* % GC [This assumes that N is large, hence the 675/N term is small] 
- The major and minor grooves of the B-form DNA helix differ by 10 A° in width. 
- Hydrogen bonds provide specificity, but not much energy. The interaction between the pi electrons on the bases provides most of the energy. 
- This is the same value as with protein folding. 
- These are positively charged amino acids that will have a favourable interaction with the negative charge on the phosphate. 
- These are amino acids that can donate and receive hydrogen bonds from the major and minor groove of the DNA. 
- All of these features can be seen on the images presented in the lecture.  
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