Feedback for Multiple Choice Quiz 31

    1. That both are required can be deduced from the energetics of each contribution. In addition, uncouplers of the proton gradient and inhibitors of the membrane potential are sufficient, separately, to stop ATP synthesis.

    2. The different affinities for ATP and ADP is the basis of the "conformational coupling" (Campbell, p. 553) that drives ATP synthesis on the rotating F1 complex.

    3. Remember that fatty acids are first activated to form an acyl-CoA. Then the chain is sequentially shortened with the release of acetyl-CoA units. See Fig. 21.6 in Campbell.

    4. Acetyl-CoA oxidation occurs only in the matrix compartment; the acyl-CoA is created in the cytosol (cytoplasm).

    5. The "balance sheet" is shown in Table 21.1.

    6. "Gotcha-type" question if you chose "8, 8". The last cycle of β-oxidation (involving a butyryl-CoA) results in two acetyl-CoA's.

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