Feedback for Multiple Choice Quiz 27 #2

  1. Pyruvate kinase uses ADP as substrate along with PEP to form ATP + pyruvate. Glucokinase and hexokinase both catalyze the phosphorylation of glucose using ATP as substrate. Phosphofructokinase catalyzes the phosphorylation of F-6-P using ATP as substrate. Adenylate kinase is not part of the glycolysis pathway.

  2. The other four choices all have relatively large -dG's. See Campbell, table 17.1 for details.

  3. The conformational change that occurs on gluccose binding prevents the hydrolysis of ATP, i.e. the futile "phosphorylation of water". (The reverse of the induced fit conformational change occurs when glucose-6-phosphate is released.)

  4. One pair of electrons is removed from glyceraldehyde-3-phosphate to form NADH and 1,3-bisphosphoglycerate (step 6). This reaction occurs twice for each glucose. Hence, 2*2 = 4 electrons total/glucose.

  5. Regeneration of NAD+ is essential for anaerobic glycolysis. Ethanol is produced by yeast, not in the human liver. NADH is used to make ATP in mitochondria under aerobic conditions.

  6. The human RBC has no organelles and relies on glycolysis for its ATP. Most common bacteria can live under aerobic and anaerobic conditions.
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