Biochemistry I Fall Term, 2004

pH Titration: A JavaScript Slideshow


Start the simulation (1 frame/sec).

This simulation shows the stepwise titration of a weak acid (AH) with a strong base to produce the anion (A-). The pH recorded is plotted vs. the equivalents of OH- added. The sizes of the symbols corresponds to the relative concentrations of AH and A-.

    Questions (you should be able to answer):
  1. What is the pKa of the weak acid in this titration?
  2. If the starting solution were 100 ml of 0.05 M AH, what volume of 1.0 M NaOH was added at each point shown above?
  3. At a ratio of AH : A- = 3 : 1, what was the measured pH?
  4. If this simulation corresponded to the titration of a phosphate salt, write the chemical structures that correspond to AH and A-
  5. . What are the other chemical species that participate in the reaction?

Back to Lecture 3: Acid-Base Equilibria & Buffers.

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8.24.04